T7902
Thermolysin from Geobacillus stearothermophilus
powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture
Sinónimos:
Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
232-973-4
MDL number:
Specific activity:
30-350 units/mg protein (E1%/280)
Biological source:
Geobacillus stearothermophilus
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Permítanos ayudarlebiological source
Geobacillus stearothermophilus
product line
BioReagent
form
powder
specific activity
30-350 units/mg protein (E1%/280)
mol wt
34.6 kDa
purified by
crystallization
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
storage temp.
−20°C
Quality Level
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Application
A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physical form
lyophilized powder containing calcium and sodium acetate buffer salts
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Allen F Ryan et al.
Frontiers in genetics, 11, 358-358 (2020-05-01)
Single-cell transcriptomics was used to profile cells of the normal murine middle ear. Clustering analysis of 6770 transcriptomes identified 17 cell clusters corresponding to distinct cell types: five epithelial, three stromal, three lymphocyte, two monocyte, two endothelial, one pericyte and
Lukas Menges et al.
British journal of pharmacology, 176(24), 4696-4707 (2019-08-20)
The intracellular signalling molecule cGMP, formed by NO-sensitive GC (NO-GC), has an established function in the vascular system. Despite numerous reports about NO-induced cGMP effects in the heart, the underlying cGMP signals are poorly characterized. Therefore, we analysed cGMP signals
David P Minde et al.
PloS one, 7(10), e46147-e46147 (2012-10-12)
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient
Natalie Seach et al.
Journal of immunological methods, 385(1-2), 23-34 (2012-08-23)
The reproducible isolation and accurate characterization of thymic epithelial cell (TEC) subsets is of critical importance to the ongoing study of thymopoiesis and its functional decline with age. The study of adult TEC, however, is significantly hampered due to the
Yuichi Kawasaki et al.
Journal of biochemistry, 153(1), 85-92 (2012-10-23)
The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stability [Yasukawa, K. and Inouye, K. (2007) Improving the activity and stability of thermolysin by site-directed mutagenesis. Biochim. Biophys. Acta 1774, 1281-1288] and
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