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34877-M

Water

HPLC Plus

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
UNSPSC Code:
12190000
NACRES:
NA.21
PubChem Substance ID:
329755083
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
MFCD00011332
Grade:
HPLC Plus
Technique(s):
HPLC: suitable
Bp:
100 °C (lit.)
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grade

HPLC Plus

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable

impurities

≤0.0003% non-volatile matter, ≤1 ppb fluorescence (quinine) at 254 nm, ≤1 ppb fluorescence (quinine) at 365 nm

refractive index

n20/D 1.34 (lit.)

pH

6-8 (25 °C)

bp

100 °C (lit.)

mp

0 °C (32 °F), 0 °C (lit.)

solubility

water: miscible

density

1.000 g/mL at 3.98 °C (lit.)

anion traces

chloride (Cl-): ≤0.1 mg/kg, fluoride (F-): ≤0.1 mg/kg, nitrate (NO3-): ≤0.1 mg/kg, sulfate (SO42-): ≤0.1 mg/kg

HPLC-gradient

≤1 mAU at 254 nm, ≤5 mAU at 210 nm

application(s)

general analytical

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

General description

HPLC Plus grade water is useful for the UHPLC (Ultra-High Pressure Liquid Chromatography) gradient elution and HPLC studies.

Application

Water has been used in the following studies:
  • To prepare the diluted hydrochloric acid solutions required for the preparation of standard solutions of amino acid.
  • In the sample extraction step during the gas chromatography mass spectrometric (GC-MS) analysis of free (FAA) and combined amino acids (CAA) in aerosol samples.
  • Preparation of tryptic digests from the standard protein samples and milk digests from milk samples.
  • Preparation of C60-aminopropylsilica.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
  • The article number 34877-4X2.5L-M will be discontinued. Please order the single bottle 34877-2.5L-M which is physically identical with the same exact specifications.
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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP5773
BCCP4220
BCCP1772
BCCP3536
BCCP6091

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Martin Fischnaller et al.
Analytica chimica acta, 761, 92-101 (2013-01-15)
Phosphorylation of proteins is an important cellular regulatory process. The analysis of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of phosphorylated species. Mass spectrometry (MS) of phosphopeptides obtained from tryptic protein digests is
Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity