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Merck

I9274

Invertase from baker′s yeast (S. cerevisiae)

200-300 units/mg solid

Synonym(s):

β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase

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About This Item

CAS Number:
9001-57-4
UNSPSC Code:
12164500
NACRES:
NA.21
EC Number:
232-615-7
MDL number:
MFCD00131410
EC Number:
3.2.1.26 (BRENDA, IUBMB)
Specific activity:
200-300 units/mg solid

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specific activity

200-300 units/mg solid

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

foreign activity

α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%, 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%

storage temp.

2-8°C

Quality Level

100

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General description

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Application

Invertase from baker′s yeast (S. cerevisiae) has been used:
  • in liquid media (YES) and is used to determine the invertase activity
  • supplemented to the reaction mixture in the extraction and analysis of sugars and starch
  • for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000475205
0000475205
0000401745
0000382426
0000369872

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Two differentially regulated mRNAs with different 5? ends encode secreted and intracellular forms of yeast invertase
Carlson M and Botstein D
Cell, 28(1), 145-154 (1982)
Carbohydrate profiles during cotton floral bud (square) development
Tarpley L and Sassenrath GF
Journal of Agromedicine, 192(5), 363-372 (2006)
Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses
Sjoblom M, et al.
Industrial Crops and Products, 74, 535-544 (2015)
Subunit structure of external invertase from Saccharomyces cerevisiae.
Trimble RB and Maley F
The Journal of Biological Chemistry, 252(12), 4409-4412 (1977)
Morphological and physiological changes in Streptomyces lividans induced by different yeasts
Santamaria R, et al.
Archives of Microbiology, 177(3), 259-266 (2002)
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