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Merck

T6399

Trichloroacetic acid

ACS reagent grade, ≥99.0%

Synonym(s):

TCA

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
200-927-2
MDL number:
Beilstein/REAXYS Number:
970119
Assay:
≥99.0%
Form:
crystalline
Grade:
ACS reagent
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Product Name

Trichloroacetic acid, ACS reagent, ≥99.0%

grade

ACS reagent

Quality Level

vapor density

<1 (vs air)

vapor pressure

1 mmHg ( 51 °C)

assay

≥99.0%

form

crystalline

impurities

Substances darkened by sulfuric acid, passes test, ≤0.01% insoluble matter

ign. residue

≤0.03%

refractive index

n20/D 1.62 (lit.)

pH

(1 at 81.7 g/l at 25 °C)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

H2O: 1 g/10 mL

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤0.002%, nitrate (NO3-): ≤0.002%, phosphate (PO43-): ≤5 ppm, sulfate (SO42-): ≤0.02%

cation traces

Fe: ≤0.001%, heavy metals (as Pb): ≤0.002%

functional group

carboxylic acid, chloro

storage temp.

2-8°C

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.
  • Trichloroacetic acid has been used for the precipitation of sLRP (soluble circulating low density lipoprotein receptor-related protein-1) isolated from plasma.
  • It has been used for the extraction of ascorbic acid from plant samples. It has also been used for the preparation of ascorbate standards for ascorbate assay.
  • It has been used for the precipitation of IMAC (immobilized metal ion affinity chromatography)-isolated phosphoproteins.


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pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup



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Measurement of reduced, oxidized and total ascorbate content in plants.
Gillespie KM and Ainsworth EA
Nature Protocols, 2, 871-871 (2007)
Casein Kinase 2 (CK2)-mediated Phosphorylation of Hsp90? as a Novel Mechanism of Rifampin-induced MDR1 Expression.
Kim SW et al.
The Journal of Biological Chemistry, 290, 17029-17029 (2015)
Impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-? is an early biomarker for mild cognitive impairment preceding Alzheimer's disease.
Sagare AP et al.
Journal of Alzheimer'S Disease, 24, 25-25 (2011)