51412C
Minimum Essential Medium
with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Minimum Essential Medium Eagle, MEM
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
description
for research or for further manufacturing use
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable, cell culture | stem cell: suitable
components
L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes
storage temp.
2-8°C
Quality Level
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Application
Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.
General description
Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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