D1806
Taq DNA Polymerase from Thermus aquaticus
with 10× PCR reaction buffer containing MgCl2
Synonym(s):
MgCl2 taq polymerase, Taq polymerase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.55
MDL number:
Biological source:
enzyme from bacterial (Thermus Aquaticus)
Recombinant:
expressed in E. coli
Concentration:
5 units/μL
biological source
enzyme from bacterial (Thermus Aquaticus)
recombinant
expressed in E. coli
form
liquid
usage
sufficient for 10000 reactions, sufficient for 3000 reactions, sufficient for 500 reactions
mol wt
94 kDa
feature
dNTPs included: no, hotstart: no, Standard PCR
Quality Level
concentration
5 units/μL
technique(s)
PCR: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR and automated sequencing reactions
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
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Application
Taq DNA Polymerase from Thermus aquaticus has been used:
- in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
- in conventional reverse transcriptase (RT)-PCR
- in simple sequence repeats (SSR) genotyping
- as a component of PCR mix for amplification of genomic and mitochondrial DNA
- in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples
Biochem/physiol Actions
Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.
Features and Benefits
- Low per unit cost of Taq
General description
Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Other Notes
One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.
Packaging
Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
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