Skip to Content
Merck

H1029

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HIS-1, monoclonal
Application:
DB, ELISA (i), IP, WB
Citations:
448

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

liquid

technique(s)

dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.

Biochem/physiol Actions

The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Suppled as ascites fluid with 15mM sodium azide.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000462557
0000462557
0000439628
0000439628
0000352386

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Association of a novel pre-ribosomal complex in Trypanosoma brucei.
Wang L
Eukaryotic Cell (2013)
A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus.
Prokunina-Olsson L, et al.
Nature Genetics, 45(2), 164-164 (2013)
Ora Hazak et al.
PLoS biology, 17(7), e3000085-e3000085 (2019-07-12)
Signaling cross talks between auxin, a regulator of plant development, and Ca2+, a universal second messenger, have been proposed to modulate developmental plasticity in plants. However, the underlying molecular mechanisms are largely unknown. Here, we report that in Arabidopsis roots
Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Savitsky D A, et al.
Proceedings of the National Academy of Sciences of the USA (2010)
G protein-coupled receptor-promoted trafficking of G?1?2 leads to AKT activation at endosomes via a mechanism mediated by G?1?2-Rab11a interaction.
Garcia-Regalado A, et al.
Molecular Biology of the Cell, 19(10), 4188-4200 (2008)
View All Related Papers

Related Content

Data Sheet - H1029

Instructions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service