L5006
Leucine Aminopeptidase, microsomal from porcine kidney
Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)
Synonym(s):
Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-942-5
MDL number:
Specific activity:
10-40 units/mg protein (Bradford)
type
Type IV-S
Quality Level
form
ammonium sulfate suspension
specific activity
10-40 units/mg protein (Bradford)
storage temp.
2-8°C
General description
Leucine Aminopeptidase belongs to the family of metalloprotease.
Application
Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.
Biochem/physiol Actions
Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.
Physical form
Suspension in 3.5 M (NH4)2SO4 solution, pH 7.7, containing 10 mM MgCl2
Other Notes
One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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QC Methods
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Barry CM, et al.
Journal of Food Science, 65(7), 1145-1150 (2000)
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