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Merck

A3010

Acylase I from porcine kidney

Grade I, lyophilized powder, ≥1500 units/mg protein

Synonym(s):

Aminoacylase, N-Acylamino acid amidohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-732-3
MDL number:
EC Number:
Specific activity:
≥1500 units/mg protein
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type

Grade I

Quality Segment

form

lyophilized powder

specific activity

≥1500 units/mg protein

composition

Protein, ≥60%

UniProt accession no.

storage temp.

−20°C

Gene Information

pig ... ACY1(396930)

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.


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pictograms

Health hazardExclamation mark

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Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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