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SRP0736

PP2A ALPHA/PPP2R1A Complex, Active

recombinant

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~38 kDa, ~65 kDa
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recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

3400-4600 nmol/min·mg (BIOMOL Green)

mol wt

~38 kDa, ~65 kDa

concentration

0.1 μg/μL

accession no.

NM_002715, NM_014225

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PP2A(5524), PPP2R1A(5518)

Application

Useful for Phosphatase assay and Western blot.

Biochem/physiol Actions

Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).

Preparation Note

Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

288-32-4

CAS No.

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