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516675 4EBP1/PHAS-I, Rat, Recombinant

4EBP1/PHAS-I, Rat, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin

516675
  
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Přehled

Replacement Information
Description
OverviewA 117-amino acid protein substrate which has been shown to be an excellent substrate for MAP kinase, p38 kinase, PKC, and JNK. Phosphorylation of PHAS-I increases in response to insulin but not to cAMP stimulation of adipocytes. An excellent substrate for MAP kinase both in vivo and in vitro. Reported to be a substrate for mammalian and yeast TOR phosphorylation.
Catalogue Number516675
Brand Family Calbiochem®
SynonymsPhosphorylated Heat- and Acid-stable Protein Regulated by Insulin
References
ReferencesAlarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.
Product Information
FormLiquid
FormulationIn 50 mM NaCl, 25 mM Tris-HCl, 5 mM β-mercaptoethanol, 500 µM EDTA, pH 7.4.
Applications
Biological Information
Biological activityShown to undergo phosphorylation by activated MAP kinase (Cat. No. 454855) in the presence of ³²P-ATP.
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
516675 0

Documentation

4EBP1/PHAS-I, Rat, Recombinant Certificates of Analysis

TitleLot Number
516675

References

Přehled odkazů
Alarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-July-2007 RFH
SynonymsPhosphorylated Heat- and Acid-stable Protein Regulated by Insulin
DescriptionA 117-amino acid protein substrate that is highly specific for MAP kinase. Phosphorylation of PHAS-I increases in response to insulin but not to cAMP stimulation of fat cells. An excellent substrate for MAP kinase both in vivo and in vitro. Reported to be a substrate for mammalian and yeast TOR phosphorylation.
FormLiquid
FormulationIn 50 mM NaCl, 25 mM Tris-HCl, 5 mM β-mercaptoethanol, 500 µM EDTA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Biological activityShown to undergo phosphorylation by activated MAP kinase (Cat. No. 454855) in the presence of ³²P-ATP.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAlarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.