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AP1014 Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb

Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)

AP1014
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~35 kDa large active subunit of caspase-9. Does not cross-react with full length caspase-9 or other cleaved caspases.
Catalogue NumberAP1014
Brand Family Calbiochem®
SynonymsAnti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)
Application Data
Detection of human Cleaved Caspase-9 by immunoblotting. Samples: Whole cell extracts from Jurkat cells (lanes 1 and 2) untreated (lane 1) or treated (lane 2) with cytochrome-c and HeLa cell extracts (lanes 3,4,5) untreated (lane 3) or treated with either staurosporine (lane 4) or cytochrome-c (lane 5). Primary antibody: Anti-Cleaved Caspase-9 (Asp315) Rabbit pAb (Cat. No. AP1014) (1:1000). Detection: chemiluminescence.
References
ReferencesMitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive controlHeLa cells treated with staurosporine
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1:100)
Application CommentsDoes not cross-react with full length caspase-9 or other cleaved caspases. For immunoblotting, incubate membrane with antibody diluted in 5% nonfat dry milk, 1X TBS, and 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³¹⁵ cleavage site of human caspase-9
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
AP1014 0

Documentation

Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb Certificates of Analysis

TitleLot Number
AP1014

References

Přehled odkazů
Mitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (1:100)
Application Data
Detection of human Cleaved Caspase-9 by immunoblotting. Samples: Whole cell extracts from Jurkat cells (lanes 1 and 2) untreated (lane 1) or treated (lane 2) with cytochrome-c and HeLa cell extracts (lanes 3,4,5) untreated (lane 3) or treated with either staurosporine (lane 4) or cytochrome-c (lane 5). Primary antibody: Anti-Cleaved Caspase-9 (Asp315) Rabbit pAb (Cat. No. AP1014) (1:1000). Detection: chemiluminescence.
DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~35 kDa large subunit of caspase-9.
BackgroundCaspase-9 is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptosis cytochrome c released from mitochondria associates with procaspase-9 (APAF, ~47 kDa) and this complex processes the procaspase-9 into a large active subunit (~17 or ~38 kDa) and a small subunit (~10 kDa) by self-cleavage at Asp315. The cleaved caspase-9 also processes other caspases, including caspases 3 and 7, to start a caspase cascade that leads to programmed cell death. Procaspase-9 can also be cleaved by caspase-3 at Asp330, serving as a positive sign to amplify the apoptotic signal in the caspase activation pathway.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³¹⁵ cleavage site of human caspase-9
IsotypeIgG
Specieshuman
Positive controlHeLa cells treated with staurosporine
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with full length caspase-9 or other cleaved caspases. For immunoblotting, incubate membrane with antibody diluted in 5% nonfat dry milk, 1X TBS, and 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.