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MABT189 Anti-Delta3 Antibody, clone 1E7.2

This Anti-Delta3 Antibody, clone 1E7.2 is validated for use in WB, FC for the detection of Delta3.

Anti-Delta3 Antibody, clone 1E7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: DLL3 UniProt Number: Q9NYJ7
MABT189
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT189
DescriptionAnti-Delta3 Antibody, clone 1E7.2
Alternate Names
  • Delta-like protein 3
  • Drosophila Delta homolog 3
  • Delta3
Background InformationDLL3, also known as Delta like ligand 3, is a transmembrane Delta-like protein principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation to inhibit primary neurogenesis. DLL3 is one of five DSL proteins that bind to the Notch receptor. Notch signaling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands family members are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in the DLL3 gene causes autosomal recessive spondylocostal dysostosis.
References
Product Information
FormatPurified
Control
  • Human liver tissue lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Delta3 Antibody, clone 1E7.2 is validated for use in WB, FC for the detection of Delta3.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected Delta3 in HEK293 cells.
Biological Information
ImmunogenLinear peptide corresponding to human Delta3.
Clone1E7.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
Gene Symbol
  • DLL3
UniProt Number
UniProt SummaryFUNCTION: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

SUBUNIT STRUCTURE: Can bind and activate Notch-1 or another Notch receptor (By similarity).

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable.

DOMAIN: The DSL domain is required for binding to the Notch receptor.

PTM: Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity).

INVOLVEMENT IN DISEASE: Defects in DLL3 are the cause of spondylocostal dysostosis type 1 (SCDO1) [MIM:277300]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.

SEQUENCE SIMILARITIES: Contains 1 DSL domain.

Contains 6 EGF-like domains.
Molecular Weight~65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Delta3 on 10 µg of human liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABT189 04053252633799

Documentation

Anti-Delta3 Antibody, clone 1E7.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Delta3 Antibody, clone 1E7.2 Certificates of Analysis

TitleLot Number
Anti-Delta3, Clone 1E7.2 - 4147720 4147720
Anti-Delta3, clone 1E7.2 3074933
Anti-Delta3, clone 1E7.2 - 2219395 2219395
Anti-Delta3, clone 1E7.2 - 3204238 3204238
Anti-Delta3, clone 1E7.2 - 3249438 3249438
Anti-Delta3, clone 1E7.2 - 3795274 3795274
Anti-Delta3, clone 1E7.2 - NRG1935580 NRG1935580
Anti-Delta3, clone 1E7.2 -2657486 2657486
Anti-Delta3, clone 1E7.2 -2710200 2710200
Anti-Delta3, clone 1E7.2 -2795668 2795668
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