ks	Katalogové číslo	Popis objednávky	ks/bal.	Cena podle ceníku
			96-Well Plate

ks	Katalogové číslo	Popis objednávky	ks/bal.	Cena podle ceníku
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

AB5724 Sigma-AldrichAnti-Dlx1 Antibody

Detect Dlx1 using this Anti-Dlx1 Antibody validated for use in WB, IH.

Anti-Dlx1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience, Neuronal & Glial Markers Gene Symbol: DLX1 UniProt Number: P56177

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Tabulka spec. kláve

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB5724
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Dlx1 Antibody
Alternate Names	Distalless

References

Product Information
Format	Affinity Purified
Presentation	Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality Level	MQ100

Applications
Application	Detect Dlx1 using this Anti-Dlx1 Antibody validated for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide, amino acids 48-60 from mouse Dlx 1.
Host	Rabbit
Specificity	Recognizes Dlx 1 (Distalless (DII) family), a homeodomain transcription factor.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001038493.1 NM_178120.4
Entrez Gene Summary	This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein is localized to the nucleus where it may function as a transcriptional regulator of signals from multiple TGF-{beta} superfamily members. The encoded protein may play a role in the control of craniofacial patterning and the differentiation and survival of inhibitory neurons in the forebrain. This gene is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 2. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene Symbol	DLX1
UniProt Number	P56177
UniProt Summary	FUNCTION: SwissProt: P56177 # Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions (By similarity). SIZE: 255 amino acids; 27320 Da SUBCELLULAR LOCATION: Nucleus (Potential). SIMILARITY: SwissProt: P56177 ## Belongs to the distal-less homeobox family. & Contains 1 homeobox DNA-binding domain.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalogové číslo	GTIN
AB5724	04053252274954

Documentation

Anti-Dlx1 Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-Dlx1 Antibody Certificates of Analysis

Title	Lot Number
RABBIT ANTI-Dlx 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2433317	2433317
RABBIT ANTI-Dlx 1 - 3440712	3440712
RABBIT ANTI-Dlx 1 - 3537148	3537148
RABBIT ANTI-Dlx 1 - 3698109	3698109
RABBIT ANTI-Dlx 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY	3008452
RABBIT ANTI-Dlx 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2276293	2276293

References

Reference overview	Pub Med ID
Gene expression relationship between prostate cancer cells of Gleason 3, 4 and normal epithelial cells as revealed by cell type-specific transcriptomes. Laura E Pascal,Ricardo Z N Vêncio,Laura S Page,Emily S Liebeskind,Christina P Shadle,Pamela Troisch,Bruz Marzolf,Lawrence D True,Leroy E Hood,Alvin Y Liu BMC cancer 9 2009 Zobrazit abstrakt Prostate cancer cells in primary tumors have been typed CD10-/CD13-/CD24hi/CD26+/CD38lo/CD44-/CD104-. This CD phenotype suggests a lineage relationship between cancer cells and luminal cells. The Gleason grade of tumors is a descriptive of tumor glandular differentiation. Higher Gleason scores are associated with treatment failure. Celý text článku	20021671
Developmental regulation of gonadotropin-releasing hormone gene expression by the MSX and DLX homeodomain protein families. Givens, ML; Rave-Harel, N; Goonewardena, VD; Kurotani, R; Berdy, SE; Swan, CH; Rubenstein, JL; Robert, B; Mellon, PL The Journal of biological chemistry 280 19156-65 2004 Zobrazit abstrakt Gonadotropin-releasing hormone (GnRH) is the central regulator of the hypothalamic-pituitary-gonadal axis, controlling sexual maturation and fertility in diverse species from fish to humans. GnRH gene expression is limited to a discrete population of neurons that migrate through the nasal region into the hypothalamus during embryonic development. The GnRH regulatory region contains four conserved homeodomain binding sites (ATTA) that are essential for basal promoter activity and cell-specific expression of the GnRH gene. MSX and DLX are members of the Antennapedia class of non-Hox homeodomain transcription factors that regulate gene expression and influence development of the craniofacial structures and anterior forebrain. Here, we report that expression patterns of the Msx and Dlx families of homeodomain transcription factors largely coincide with the migratory route of GnRH neurons and co-express with GnRH in neurons during embryonic development. In addition, MSX and DLX family members bind directly to the ATTA consensus sequences and regulate transcriptional activity of the GnRH promoter. Finally, mice lacking MSX1 or DLX1 and 2 show altered numbers of GnRH-expressing cells in regions where these factors likely function. These findings strongly support a role for MSX and DLX in contributing to spatiotemporal regulation of GnRH transcription during development.	15743757

Reference overview

Pub Med ID

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Kategorie

Life Science Research > Antibodies and Assays > Primary Antibodies

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