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ST1115 Anti-PDK1 (1-556) Rabbit pAb

Anti-PDK1 (1-556) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phosphoinositide-Dependent Protein Kinase-1

ST1115
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~60 kDa PDK1 protein in HEK293 cells. Also recognizes ~400 kDa and ~17 kDa unidentified proteins.
Catalogue NumberST1115
Brand Family Calbiochem®
SynonymsAnti-Phosphoinositide-Dependent Protein Kinase-1
Application Data
Detection of human PDK1 by immunoblotting. Samples: full-length, recombinant human PDK1 (lane 2) and whole cell lysate (40µg) from HEK293 cells (lane 3). Primary antibody: Anti-PDK1 (1-556) Rabbit pAb (1:3000). Detection: chemiluminescence. (Lane 1: MWM).
References
ReferencesFeldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlHEK293 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:3000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenfull-length, recombinant, human PDK1
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1115 0

Documentation

References

Přehled odkazů
Feldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.

Brochure

Title
Diabetes and Obesity
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-October-2007 RFH
SynonymsAnti-Phosphoinositide-Dependent Protein Kinase-1
ApplicationImmunoblotting (1:3000)
Application Data
Detection of human PDK1 by immunoblotting. Samples: full-length, recombinant human PDK1 (lane 2) and whole cell lysate (40µg) from HEK293 cells (lane 3). Primary antibody: Anti-PDK1 (1-556) Rabbit pAb (1:3000). Detection: chemiluminescence. (Lane 1: MWM).
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~60 kDa PDK1 protein.
Background3-phosphoinositide-dependent kinase 1 (PDK1; accession no. NP-002613) is a serin/threonine protein kinase that plays a major role in cell signaling pathways, cancer, cell growth, and tumorigenesis. PDK1 consist of a N-terminal kinase domain and a C-terminal pleckstrin homology (PH) domain. The PH domain binds to the PI 3-K products, PIP3 and PIP2, which target PDK1 to the plasma membrane where it phosphorylates AKT at Thr308. PDK1 can also directly associate and phosphorylate many other important substrates including PKC, p70S6K, and SGK. PDK1 is activated by phosphorylation at Ser241. Recently, an autonomous nuclear-specific PI 3-K signaling pathway has been discovered that contains all of the key PI 3-K signaling components including AKT, PKC, and p70S6K. PDK1 has also been found in the nucleus, suggesting that this protein is shuttled from the cytoplasm to the nucleus.
HostRabbit
Immunogen speciesHuman
Immunogenfull-length, recombinant, human PDK1
IsotypeIgG
Specieshuman
Positive controlHEK293 cells
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesFeldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.