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SCT215 BioTracker™ 497 Green Actin Live Cell Probe

Live cell imaging cytoskeleton probe used to detect actin using fluorescence microscopy or flow cytometry.

SCT215
5 vials  
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue NumberSCT215
DescriptionBioTracker™ 497 Green Actin Live Cell Probe
OverviewThe BioTracker™ 497 Green Actin Live Cell Probe may be used in both living cells and fixed-cell applications. Because SCT215 is an organic fluorescent small molecule with ready membrane permeability, it enables visualization of intracellular actin filamentsby simple addition of the probe to the cell culture medium or extracellular fluid without a rinsing step.

This probe is thought to bind to F-actin through the common binding site for phalloidin and jasplakinolide.

Spectral Properties
Absorbance (max): 497 nm
Emission (max): 525 nm (Orange)
Alternate Names
  • Live Cell Imaging Probe
  • Live Cell Actin Probe
  • Green Live Cell Actin Probe
Background InformationThe BioTracker™ 497 Green Actin Live Cell Probe is a fluorescent probe that specifically binds to actin filaments (F-actin) and can visualize F-actin with green fluorescence.
References
Product Information
Detection methodFluorescence Flow cytometry
FormatLyophilized solid
PresentationLyophilized. Orange solid.
Quality LevelMQ100
Applications
ApplicationLive cell imaging cytoskeleton probe used to detect actin using fluorescence microscopy or flow cytometry.
Key Applications
  • Cell Based Assays
  • Flow Cytometry
  • Fluorescence
Application NotesSCT215 fluorescence can
be observed with a blue excitation
and green fluorescence filter set for GFP/FITC. For
live-cell imaging, use lower concentrations of
probe, weaken the excitation intensity and use
high-sensitivity cameras to reduce exposure time.
Consider the use of antifade reagent for time-lapse
or continuous imaging.
Stock solution is prepared by adding 30 uL DMSO to one vial,, which results in a pale yellow liquid.
Diluting stock solution in culture medium to 50-100 nM before adding to cells is recommended, although optimal incubation concentraiton may be determined according to user application. Use the lowest possible concentration to limit cytotoxicity.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ 497 Green Actin Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. After dissolving in DMSO, store aliquots at -20 °C.
Packaging Information
Material Size5 vials
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
SCT215 04065269233329