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324825 EPAC2 cAMP Binding Domain, GST-Fusion, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
324825
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human EPAC2 (Exchange Protein dirctly Activated by cAMP) consisting of amino acids 302-470 expressed in E. coli. EPAC2 is a guanine nucleotide exchange factor (GEF) that activates Rap GTPase upon bing to cAMP. Supplied as a partially purified preparation of catalytically active enzyme useful for binding and activity assays.
Catalogue Number324825
Brand Family Calbiochem®
Application Data
cAMP binding to EPAC2
References
ReferencesBos, J.L., 2006. Trends Biochem Sci. 31, 680.
McPhee. I., et al. 2005. Biochem Soc Trans. 33, 1330.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Applications
Biological Information
Biological activityEC₅₀ of cAMP binding: ≤1.5 µM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
324825 0

Documentation

EPAC2 cAMP Binding Domain, GST-Fusion, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
324825

References

Přehled odkazů
Bos, J.L., 2006. Trends Biochem Sci. 31, 680.
McPhee. I., et al. 2005. Biochem Soc Trans. 33, 1330.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-June-2010 JSW
Application Data
cAMP binding to EPAC2
DescriptionRecombinant, human EPAC2 (Exchange Protein dirctly Activated by cAMP) consisting of amino acids 302-470 expressed in E. coli. EPAC2 is a guanine nucleotide exchange factor (GEF) that activates Rap GTPase upon binding to cAMP. Supplied as a partially purified preparation of catalytically active enzyme useful for binding and activity assays.
FormLiquid
FormulationIn 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Recommended reaction conditions
Activity and Inhibition Assay

Materials Required •EPAC Buffer: 50 mM Tris, 50 mM NaCl, 5% Glycerol, pH 7.4 •96 well plates: Griener 655075, white •Ysi beads: Perkin Elmer RPNQ0033, stock concentration of 50 mg/5ml in 50 mM Tris, 0.1% Sodium Azide, pH 7.4 • 3H-cAMP • Compound of choice for inhibition: Dilute to desired concentration.
Protocol 1. Perform serial dilutions of EPAC2 protein to determine optimum concentration per well. 2. Make a mixture of EPAC2 protein and EPAC Buffer to a total volume of 4 ml. The ratio depends on amount of protein added; i.e. 200µl EPAC2 protein and 3.8 ml EPAC Buffer/100µg Ysi beads (1 ml/plate). 3. Make 3H-cAMP to 1.5µCi/ml in EPAC Buffer. 4. Add 11µl compound. 5. Add 50µl EPAC2 protein/EPAC buffer/ 10 µg Ysi beads, while shaking to stop the beads from settling. 6. Add 50µl 3H-cAMP. 7. Incubate on shaker for 30 min. 8. Read on a scintillation counter.
Biological activityEC₅₀ of cAMP binding: ≤1.5 µM
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesBos, J.L., 2006. Trends Biochem Sci. 31, 680.
McPhee. I., et al. 2005. Biochem Soc Trans. 33, 1330.