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PS1019 PhosphoDetect™ Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

This PhosphoDetect™ Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr183/185)

PS1019
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Katalogové čísloBalení ks/bal.
PS1019-100UL Plastová ampulka 100 ul
Description
OverviewRecognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr183 and Tyr185 in uv-treated HEK293 cells.
Catalogue NumberPS1019
Brand Family Calbiochem®
SynonymsAnti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr183/185)
Application Data
Detection of human JNK1 and JNK2, phosphorylated at Thr183 and Tyr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-JNK1&2 (pThr183/pTyr185 Rabbit pAb (Cat. No. PS1019) (1:1000) pre-incubated without peptide (lanes 1,6), with the phosphopeptide immunogen (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesChoudhury, B.K., et al. 2002. J. Immunol. 169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol. 33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol. 21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol. 21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol. 11, 426.
Tong, T., et al. 2001. Exp. Cell Res. 269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol. 71, 479.
Basu, S., et al. 1998. Oncogene 17, 3277.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
Positive controlUV-treated HEK293 cells or sorbitol-treated PC12 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PS1019-100UL 04055977227109

Documentation

PhosphoDetect™ Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb Certificates of Analysis

TitleLot Number
PS1019

References

Přehled odkazů
Choudhury, B.K., et al. 2002. J. Immunol. 169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol. 33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol. 21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol. 21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol. 11, 426.
Tong, T., et al. 2001. Exp. Cell Res. 269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol. 71, 479.
Basu, S., et al. 1998. Oncogene 17, 3277.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 RFH
SynonymsAnti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr183/185)
ApplicationImmunoblotting (1:1000)
Application Data
Detection of human JNK1 and JNK2, phosphorylated at Thr183 and Tyr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-JNK1&2 (pThr183/pTyr185 Rabbit pAb (Cat. No. PS1019) (1:1000) pre-incubated without peptide (lanes 1,6), with the phosphopeptide immunogen (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr183 and Tyr185.
Backgroundc-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/MAP Kinase (SAPK/MAPK) that exists as three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is activated as a result of phosphorylation on Thr183 and Tyr185 by MEK4 and MEK7. JNK1 and JNK2 are expressed by numerous cells and tissues, while JNK3 is primarily expressed in neuronal cells. The JNK pathway, along with the p38 pathway, functions to modulate cell cycle, apoptotic, and transcriptional responses to stress.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2
IsotypeIgG
Specieshuman, mouse, rat
Positive controlUV-treated HEK293 cells or sorbitol-treated PC12 cells
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChoudhury, B.K., et al. 2002. J. Immunol. 169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol. 33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol. 21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol. 21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol. 11, 426.
Tong, T., et al. 2001. Exp. Cell Res. 269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol. 71, 479.
Basu, S., et al. 1998. Oncogene 17, 3277.