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The PureGenome™ Low Input NGS Library Construction Kit streamlines the process of generating indexed, or barcoded libraries, for Illumina® HiSeq® sequencing from extremely low amounts of input DNA (from 50 pg to 1 ng). This kit is well suited for applications such as ChIP-Seq (chromatin immunoprecipitation followed by next generation sequencing) as well as other sequencing applications where input DNA is limited.
To achieve this level of sensitivity, the PureGenome™ Low Input NGS kit utilizes a unique technology that adds adaptors to sheared genomic DNA with ultra-high efficiency. This methodology enables the previous limits of NGS library construction to be reduced to below 1 ng. Additionally, this approach greatly simplifies NGS library construction. As compared to conventional approaches that require DNA isolation between protocol steps, the PureGenome™ Low Input NGS process occurs in a single tube and does not require any in process DNA isolation steps. Once library construction is complete, in a final step, DNA purification and size selection is performed using the Agencourt® AMPure® XP beads provided with this kit.
Sensitive: Generate libraries with as little as 50 pg of input DNA
Fast: Construct instrument ready libraries in about 2 hours
Convenient: Single tube repair, ligation, and amplification steps & no DNA purification step required.
Robust: High diversity, low duplicate reads.
Proven: Demonstrated performance in ChIP-seq library construction
Reproducible: Get reproducible libraries across 50pg – 50ng range
Complete: All key reagents provided including 12 Illumina indexing barcodes and Agencourt® AMPure® XP beads for recovery and concentration of final library.