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RAB0149

Human EGF ELISA Kit

Name: Human EGF ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatant.

Synonym(s):

EGF ELISA Kit

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About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... EGF(1950)

Input:

sample type cell culture supernatant(s)
sample type plasma
sample type serum

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.82-200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGF(1950)

Description

General description

The Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGF in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Human EGF

Application

Human EGF ELISA Kit has been used to examine the concentration of epidermal growth factor (EGF) in adipose derived stem cell conditioned medium (ADSC-CM).

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, which binds to EGF receptor. EGF increases proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It blocks gastric acid and pepsin secretion and enhances gastrointestinal cell proliferation. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is associated with hypomagnesemia..

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

RABELADA
ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
SDS

RABELADB
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
SDS

RABSTOP3
ELISA Stop Solution (Item I)
SDS

RABTMB3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
SDS

RABWASH4
20X Wash Buffer (Item B)
SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number

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