05-1336
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312
clone CMA312, from mouse
Synonym(s):
H3S10P, Histone H3 (phospho S10), H3 histone, family 3A
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
CMA312, monoclonal
Species reactivity:
vertebrates, human
Application:
ChIP, ELISA, ICC, WB, multiplexing
Technique(s):
ChIP: suitable, ELISA: suitable, immunocytochemistry: suitable, multiplexing: suitable, western blot: suitable
Citations:
20
Uniprot accession no.:
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
CMA312, monoclonal
species reactivity
vertebrates, human
technique(s)
ChIP: suitable, ELISA: suitable, immunocytochemistry: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Quality Level
Gene Information
human ... H3C1(8350)
Analysis Note
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Application
ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
Biochem/physiol Actions
Broad species cross-reactivity is expected, based on sequence homology.
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
~17 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1093
Physical form
Format: Purified
Legal Information
Luminex is a registered trademark of Luminex Corp
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Anna Alexovič Matiašová et al.
The Journal of comparative neurology, 525(3), 693-707 (2016-08-10)
According to previous opinion, the derivation of neurons and glia from the central canal (CC) lining of the spinal cord in rodents should occur in the embryonic period. Reports of the mitotic activity observed in the lining during postnatal development
Manjit Singh et al.
The Plant cell, 23(2), 443-458 (2011-02-18)
Apomixis is a form of asexual reproduction through seeds in angiosperms. Apomictic plants bypass meiosis and fertilization, developing offspring that are genetically identical to their mother. In a genetic screen for maize (Zea mays) mutants mimicking aspects of apomixis, we
Elizabeth N Schock et al.
PloS one, 12(3), e0174206-e0174206 (2017-03-28)
Primary cilia are nearly ubiquitous, cellular projections that function to transduce molecular signals during development. Loss of functional primary cilia has a particularly profound effect on the developing craniofacial complex, causing several anomalies including craniosynostosis, micrognathia, midfacial dysplasia, cleft lip/palate
Roswitha Nast et al.
Frontiers in immunology, 11, 581241-581241 (2020-10-20)
Host defense against the human pathogen Toxoplasma gondii depends on secretion of interferon (IFN)-γ and subsequent activation of monocytic cells to combat intracellular parasites. Previous studies have shown that T. gondii evades IFN-γ-mediated immunity by secreting the effector TgIST into
Hong Liu et al.
Nature cell biology, 15(1), 40-49 (2012-12-18)
Timely dissolution of sister-chromatid cohesion in mitosis ensures accurate chromosome segregation to guard against aneuploidy and tumorigenesis. The complex of shugoshin and protein phosphatase 2A (SGO1-PP2A) protects cohesin at centromeres from premature removal by mitotic kinases and WAPL in prophase.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-1336 | 04053252512711 |
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