69839-M
T4 DNA Ligase - Novagen
Qualified under the most stringent cloning conditions
Synonym(s):
DNA Ligation
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About This Item
NACRES:
NA.54
UNSPSC Code:
41106310
Storage temp.:
−20°C
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
dry ice, wet ice
storage temp.
−20°C
General description
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.
Other Notes
4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Norit is a registered trademark of Norit N.V.
Disclaimer
Toxicity: Standard Handling
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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