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Merck

AB9206

Anti-DARPP-32 Antibody, phosphoThr34

Chemicon®, from rabbit

Synonym(s):

Anti-DARPP-32, Anti-DARPP32

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-DARPP-32 Antibody, phosphoThr34, Chemicon®, from rabbit

biological source

rabbit

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

NM_032192.2
NM_181505.2

UniProt accession no.

Q9UD71

shipped in

dry ice

target post-translational modification

phosphorylation (pThr34)

Quality Level

100

Gene Information

human ... PPP1R1B(84152)

Analysis Note

Control
Cultured primary neurons, rat brain lysate and human breast adenocarcinoma

Application

Research Category
Neuroscience
Research Sub Category
Reward & Addiction

Signaling Neuroscience
This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.
Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes DARPP-32 phosphorylated at threonine 34 .

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

~32 kD

Immunogen

Epitope: phosphoThr34
Synthetic peptide from DARPP-32, phosphoThr34.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.
ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lídia Cantacorps et al.
British journal of pharmacology, 177(5), 1090-1105 (2019-11-11)
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Nature communications, 12(1), 3495-3495 (2021-06-11)
Lysosomal storage disorders characterized by altered metabolism of heparan sulfate, including Mucopolysaccharidosis (MPS) III and MPS-II, exhibit lysosomal dysfunctions leading to neurodegeneration and dementia in children. In lysosomal storage disorders, dementia is preceded by severe and therapy-resistant autistic-like symptoms of

Global Trade Item Number

SKUGTIN
AB920604053252626210

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