43925
Nigrosin Stain solution
suitable for microscopy
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
suitability
suitable for microscopy, Bacillus spp., Corynebacterium spp.
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
General description
Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.
Other Notes
Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water
Nigrosin 100 g/l, Formalin 5 ml/l in water
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
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Leonardo F C Brito et al.
Theriogenology, 60(8), 1539-1551 (2003-10-02)
The objectives of this study were to compare different methods of evaluating sperm plasmalemma and to determine their relationship with in vitro fertilization rate. A single batch of frozen semen from each of eight beef bulls was used for assessment
D Kutter et al.
Clinica chimica acta; international journal of clinical chemistry, 258(2), 231-239 (1997-02-17)
Nigrosin binds to proteins yielding a dark blue complex. This reaction is used for detecting low protein concentrations in urine by applying spots of urine to cellulose acetate strips which are coloured by nigrosin. The intensity of coloured spots remaining
Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a
K Matsuda et al.
Journal of clinical laboratory analysis, 15(4), 171-174 (2001-07-04)
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining
D A Hewitt et al.
Animal reproduction science, 51(4), 321-332 (1998-08-01)
The processes of capacitation and acrosomal exocytosis of dog spermatozoa in vitro have yet to be fully investigated. Firstly, we investigated the effectiveness of a technique for staining dog spermatozoa with the fluorescent labels Hoechst 33258 and chlortetracycline. A modified
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