product line
BioReagent
Quality Level
assay
≥90% (HPLC), ≥90% (degree of coupling )
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV absorption
λ: 501-506 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.
Application
Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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