48275
β-Galactosidase from Escherichia coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
~140 U/mg
form
powder
Quality Level
quality
lyophilized
specific activity
~140 U/mg
mol wt
Mr ~135000
contains
phosphate buffer as stabilizer, sucrose as stabilizer
technique(s)
enzyme immunoassay: suitable
shipped in
wet ice
storage temp.
−20°C
General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590) ; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique ; Enzyme immunoassay of thyrptropin releasing hormone (TRH) ; Preparation of enzyme-antibody conjugates.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates