A0855
Anti-Auxin antibody, Mouse monoclonal
clone 1E11-C11, purified from hybridoma cell culture
Synonym(s):
Anti-IAA, Anti-Indole-3-acetic acid
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About This Item
Conjugate:
unconjugated
Clone:
1E11-C11, monoclonal
Application:
DB, ELISA (i), IF, IHC, WB
Citations:
15
biological source
mouse
Quality Level
recombinant
expressed in mouse cell line
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1E11-C11, monoclonal
form
PBS solution
species reactivity
plant
technique(s)
dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA, western blot: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.
Immunogen
indoleacetic acid (IAA) carboxyl linked to ovalbumin.
Application
Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.
Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Related Content
Tadashi Sakata et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(19), 8569-8574 (2010-04-28)
With global warming, plant high temperature injury is becoming an increasingly serious problem. In wheat, barley, and various other commercially important crops, the early phase of anther development is especially susceptible to high temperatures. Activation of auxin biosynthesis with increased
Yolanda Pérez-Pérez et al.
International journal of molecular sciences, 24(13) (2023-07-14)
The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present
Aurora Alaguero-Cordovilla et al.
Plant, cell & environment, 44(5), 1642-1662 (2021-01-20)
Adventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum