A2580
Angiotensin Converting Enzyme from porcine kidney
lyophilized powder, ≥10 units/mg protein (Bradford)
Synonym(s):
ACE, Peptidyl-dipeptidase A
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About This Item
CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Specific activity:
≥10 units/mg protein (Bradford)
form
lyophilized powder
specific activity
≥10 units/mg protein (Bradford)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
pig ... ACE(613133)
General description
ACE is a monomer with molecular weight of ~170 kDa
pH range for activity: 7-8.5
Temperature optimum: 37 °C
Zinc is required for activity
Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM)
pH range for activity: 7-8.5
Temperature optimum: 37 °C
Zinc is required for activity
Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM)
Angiotensin converting enzyme (ACE) is encoded by the gene, mRNA-decapping enzyme subunit 1 (DCP1). It is a zinc metalloprotease, which belongs to the M2 family.
Application
Angiotensin converting enzyme from porcine kidney has been used in in vitro angiotensin-converting enzyme (ACE) inhibition assay.
Biochem/physiol Actions
Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II, which regulates the fluid-electrolyte balance and systemic blood pressure. ACE inhibits the vasodilator, bradykinin. ACE inhibitors are used to treat high blood pressure.
Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.
Physical form
Lyophilized powder containing Tris buffer salts.
Other Notes
One unit will produce 1.0 ·μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Sequence variation in the human angiotensin converting enzyme
Rieder MJ, et al.
Nature Genetics, 22(1), 59-59 (1999)
Effect of angiotensin-converting-enzyme inhibition compared with conventional therapy on cardiovascular morbidity and mortality in hypertension: the Captopril Prevention Project (CAPPP) randomised trial
Hansson L, et al.
Lancet, 353(9153), 611-616 (1999)
Rodrigo A Fraga-Silva et al.
Hypertension (Dallas, Tex. : 1979), 61(6), 1233-1238 (2013-04-24)
Diminished release and function of endothelium-derived nitric oxide coupled with increases in reactive oxygen species production is critical in endothelial dysfunction. Recent evidences have shown that activation of the protective axis of the renin-angiotensin system composed by angiotensin-converting enzyme 2
Prolyl carboxypeptidase: a forgotten kidney angiotensinase. Focus on "Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry".
Juan Carlos Q Velez
American journal of physiology. Cell physiology, 304(10), C939-C940 (2013-04-05)
A Human Homolog of Angiotensin Converting Enzyme-Cloning and Functional Expression As A Captopril Insensitive Carboxypeptidase
Tipnis SR, et al.
The Journal of Biological Chemistry (2000)
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