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C0875

Calcein

Name: Calcein
Brand: SIGMA

Powder

Synonym(s):

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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About This Item

Empirical Formula (Hill Notation):

C₃₀H₂₆N₂O₁₃

CAS Number:

154071-48-4

Molecular Weight:

622.53

MDL number:

MFCD00005049

UNSPSC Code:

12171500

PubChem Substance ID:

24892279

NACRES:

NA.47

Beilstein/REAXYS Number:

8181068

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Properties

Product Name

Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Quality Level

200

description

mixed isomers

form

powder

technique(s)

titration: suitable

color

yellow to brown

solubility

NaOH: 50 mg/mL

fluorescence

λ_ex 470 nm; λ_em 509 nm (pH 7.4)

suitability

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

InChI key

DEGAKNSWVGKMLS-UHFFFAOYSA-N

Description

Application

Calcein has been used:

in whole cell staining
in histomorphometry and micro-computed tomography
to label active bone-forming surfaces

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000552273

SHBT2898

0000318477

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Calcium: Chemistry, Analysis, Function and Effects (2015)

Skeletal abnormalities in Pth-null mice are influenced by dietary calcium.

Miao D, et al.

Endocrinology, 145, 2046-2046 (2004)

Parathyroid hormone-related peptide is required for increased trabecular bone volume in parathyroid hormone-null mice.