E4274
Endotoxin Removal solution
Remove lipopolysaccharides (LPS) from plasmid DNA preparations
Synonym(s):
ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free
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About This Item
NACRES:
NA.52
UNSPSC Code:
12352200
Quality Level
Application
Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.
Biochem/physiol Actions
The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.
Preparation Note
1 volume treats 5 volumes of DNA solution (≤1mg/ml)
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Datasheet
Determination of endotoxin through an aptamer-based impedance biosensor.
Su W
Biosensors And Bioelectronics, 32(1), 32-36 (2012)
M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)