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H3537

HEPES solution

Name: HEPES solution
Brand: SIGMA

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Synonym(s):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

CAS Number:

7365-45-9

UNSPSC Code:

12161700

PubChem Substance ID:

329815199

NACRES:

NA.25

MDL number:

MFCD00006158

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Properties

Product Name

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm, heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:

To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
To recover purified ribonucleotide
As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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