L0520

Lactoferrin from human milk

Name: Lactoferrin from human milk
Brand: SIGMA

≥85% (SDS-PAGE), lyophilized powder

Synonym(s):

Growth-inhibiting protein 12, Lactotransferrin, Talalactoferrin

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About This Item

CAS Number:

936541-36-5

UNSPSC Code:

12352202

NACRES:

NA.61

MDL number:

MFCD00164307

Product Name

Lactoferrin from human milk, ≥85% (SDS-PAGE), lyophilized powder

SMILES string

N([C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)O)C(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)CC(=O)N)C)CC(=O)O

InChI

1S/C35H53N9O12/c1-16(2)27(43-33(53)23(14-26(47)48)41-25(46)15-38-29(49)18(5)39-31(51)21(36)13-24(37)45)34(54)40-19(6)30(50)42-22(12-20-10-8-7-9-11-20)32(52)44-28(17(3)4)35(55)56/h7-11,16-19,21-23,27-28H,12-15,36H2,1-6H3,(H2,37,45)(H,38,49)(H,39,51)(H,40,54)(H,41,46)(H,42,50)(H,43,53)(H,44,52)(H,47,48)(H,55,56)/t18-,19-,21-,22-,23-,27-,28-/m0/s1

InChI key

QCBUWCQOKPLTDZ-PKRULZLPSA-N

biological source

human milk

assay

≥85% (SDS-PAGE)

form

lyophilized powder

mol wt

82.4 kDa

composition

Protein, ≥90% biuret

technique(s)

microbiological culture: suitable

solubility

phosphate buffer: 1 mg/mL, clear to slightly hazy (0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C)

UniProt accession no.

P02788

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... LTF(4057)

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Analysis Note

Contains iron, see certificate of analysis for lot specific information.

Application

Lactoferrin was used to grow Streptococcus mutans in an iron-limiting medium. It was used to test if lactoferrin impedes epithelial cell adhesion in vitro.

Biochem/physiol Actions

Lactoferrin is an iron binding protein. It is structurally similar to transferrin, the plasma iron transport protein; but lactoferrin has a much higher affinity for iron (250 fold). It is very abundant in colostrum and small amounts can also be found in tears, saliva, mucous secretions and in the secondary granules of neutrophils. It is made by mucosal epithelium and neutrophils and is released by these cells in response to inflammatory stimuli. Bacterial growth is inhibited by its ability to sequester iron and also permeabilize bacterial cell walls by binding to lipopolysaccharides through its N-terminus. Lactoferrin can inhibit viral infection by binding tightly to the viral envelope protein. This prevents cell-virus fusion by blocking the binding domain. Lactoferrin appears to activate host defense systems in part by stimulating the release of interleukin-8, a neutrophil activator. It may also be involved in antibody and interleukin synthesis, lymphocyte proliferation and complement activation.

Preparation Note

Chromatographically purified.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Glycan involvement in the adhesion of Pseudomonas aeruginosa to tears.

Liisa Kautto et al.

Experimental eye research, 145, 278-288 (2016-02-07)

The human eye is constantly bathed by tears, which protect the ocular surface via a variety of mechanisms. The O-linked glycans of tear mucins have long been considered to play a role in binding to pathogens and facilitating their removal

Lactoferrin is a dynamic protein in human melioidosis and is a TLR4-dependent driver of TNF-α release in Burkholderia thailandensis infection in vitro.

Shelton W Wright et al.

PLoS neglected tropical diseases, 14(8), e0008495-e0008495 (2020-08-09)

Melioidosis is an often-severe tropical infection caused by Burkholderia pseudomallei (Bp) with high associated morbidity and mortality. Burkholderia thailandensis (Bt) is a closely related surrogate that does not require BSL-3 conditions for study. Lactoferrin is an iron-binding glycoprotein that can

Lactoferrin restrains allergen-induced pleurisy in mice.

Michał Zimecki et al.

Inflammation research : official journal of the European Histamine Research Society ... [et al.], 61(11), 1247-1255 (2012-07-20)

The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice. BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used. Mice were immunized intraperitoneally

Acinetobacter baumannii can use multiple siderophores for iron acquisition, but only acinetobactin is required for virulence.

Jessica R Sheldon et al.

PLoS pathogens, 16(10), e1008995-e1008995 (2020-10-20)

Acinetobacter baumannii is an emerging pathogen that poses a global health threat due to a lack of therapeutic options for treating drug-resistant strains. In addition to acquiring resistance to last-resort antibiotics, the success of A. baumannii is partially due to

Augmentation of Urinary Lactoferrin Enhances Host Innate Immune Clearance of Uropathogenic Escherichia coli.

Kathryn A Patras et al.

Journal of innate immunity, 11(6), 481-495 (2019-05-06)

Urinary tract infection (UTI) is a prominent global health care burden. Although UTI is readily treated with antibiotics in healthy adults, complicated cases in immune-compromised individuals and the emerging antibiotic resistance of several uropathogens have accelerated the need for new

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