M3821

Anti-Myelin Basic Protein Antibody

Name: Anti-Myelin Basic Protein Antibody
Brand: SIGMA

rabbit polyclonal

Synonym(s):

Anti-MBP

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01322361

Product Name

Anti-Myelin Basic Protein (MBP) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 17-22 kDa (isoform)
antigen ~22 kDa (isoform)
antigen ~33 kDa (isoform)

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

UniProt accession no.

P02686

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)

Related Categories

Flow Cytometry Antibodies

Application

Anti-Myelin Basic Protein (MBP) antibody produced in rabbit has been used in:

indirect immunolabeling
immunohistochemistry.
Western Blotting.

Anti-myelin basic protein (MBP) antibody (diluted 1:200) can be used as a primary antibody in double fluorescence labeling. It can also be used to detect myelin basic protein using immunoblotting.

Biochem/physiol Actions

Myelin basic protein (MBP) plays a vital role in formation of myelin sheath and is associated with the various neurological disorders. MBP functions as an antigenic target in autoimmune disease experimental allergic encephalomyelitis (EAE). MBP plays an essential role in cytoskeletal turnover and membrane extension. Additionally, it is also involved in Fyn-mediated signaling pathways, sequestration of phosphoinositides and regulation of calcium homoeostasis. MBP functions as an in vitro substrate for mitogen-activated protein kinase (MAP) kinase and aids in the purification and functional studies of enzyme.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myelin Basic Protein (MBP) is a hydrophilic protein that is expressed mainly in oligodendrocytes. It has a pivotal role in myelination of nervous system, signaling pathways prior to myelination and remyelination processes after neural injury. Anti-myelin basic protein (MBP) antibody can be used in western blotting and flow cytometry. Rabbit anti-myelin basic protein antibody reacts specifically with myelin basic protein of mouse, rat and human.

Myelin basic protein (MBP) is an 18.5kDa protein encoded by the gene mapped to human chromosome 18q22qter. MBP is a major component of the myelin sheath and is highly expressed in central nervous system (CNS).

Immunogen

synthetic peptide corresponding to residues 102-116 of human Myelin Basic Protein.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.

Erickson AK, et al.

The Journal of Biological Chemistry, 265, 19728-19735 (1990)

Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve.

Nishimoto S, et al.

Frontiers in Cellular Neuroscience (2015)

The Axonal Guidance Cue Semaphorin 3C Contributes to Alveolar Growth and Repair

Vadivel A, et al.

PLoS ONE, 8 (2013)

Central nervous system myelin: structure, function, and pathology.

C M Deber et al.

Clinical biochemistry, 24(2), 113-134 (1991-04-01)

Multiple sclerosis (MS) and a number of related distinctive diseases are characterized by the active degradation of central nervous system (CNS) myelin, an axonal sheath comprised essentially of proteins and lipids. These demyelinating diseases appear to arise from complex interactions

Functional motor recovery from motoneuron axotomy is compromised in mice with defective corticospinal projections.

Ding Y, et al.

PLoS ONE, 9 (2014)

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