M5904
Mineral oil
BioReagent, Molecular Biology, light oil
Synonym(s):
light mineral oil
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About This Item
Grade:
Molecular Biology
Technique(s):
PCR: suitable
grade
Molecular Biology
Quality Level
product line
BioReagent
form
light oil
technique(s)
PCR: suitable
color
colorless
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
suitability
suitable for overlaying aqueous reactions and centrifuge gradients
foreign activity
DNase, RNase, Protease, none detected
storage temp.
room temp
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
General description
Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.
Application
Mineral oil has been used:
- to suspend the gels for capillary contraction assay
- in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
- as a continuous phase to prevent droplet coalescence in emulsification system
Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.
Biochem/physiol Actions
Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves
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Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated
Electrical distribution and installation
Plant Engineer's Reference Book (2002)