N6658
Nutrient Mixture F-12 Ham
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Ham’s F-12
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About This Item
Product Name
Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.
Application
Nutrient Mixture F-12 Ham has been used:
- to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
- to culture LAN-1 cells
- to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
- with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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