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Merck

O2260

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

Synonym(s):

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD03100698
Biological source:
mouse
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Mol wt:
30 kDa by SDS-PAGE (reducing)
 65 kDa by SDS-PAGE (reducing) (doublet)
Assay:
>95% (SDS-PAGE)

Key Documents

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Product Name

Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

biological source

mouse

recombinant

expressed in NSO cells

sterility

sterile

assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

30 kDa by SDS-PAGE (reducing)
 65 kDa by SDS-PAGE (reducing) (doublet)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P10923

shipped in

dry ice

storage temp.

−20°C

Quality Level

100

Gene Information

mouse ... Spp1(20750)

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Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Application

Osteopontin from mouse has been used:
  • to study its role in a mouse model of preterm brain injury
  • as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
  • to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Biochem/physiol Actions

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX0946
MKCW1227
MKCV8841
MKCT8136
MKCS5479

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Osteopontin upregulation in atherogenesis is associated with cellular oxidative stress triggered by the activation of scavenger receptors
Jimenez CAE, et al.
Archives of Medical Research, 43(2), 102-111 (2012)
Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival
Denhardt DT, et al.
The Journal of Clinical Investigation, 107(9), 1055-1061 (2001)
The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
Albertsson AM, et al.
Journal of Neuroinflammation, 11(1), 197-197 (2014)
Effect of osteopontin, chondroitin sulfates (B, C), and human serum albumin on the crystallization behavior of hydroxyapatite in agarose and silica hydrogels
Jimenez CAE, et al.
Crystal Growth & Design, 8(4), 1335-1339 (2008)
Yunan Zhu et al.
Japanese journal of infectious diseases, 68(6), 467-473 (2015-04-14)
The level of osteopontin (OPN) increases during bacterial lung infection. However, the OPN level in virus-induced lung injury is unclear, and the relationship between the hyer-production of OPN and lung injury remains to be thoroughly understood. Therefore, we sought to
View All Related Papers

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