P7127

Phospho(enol)pyruvic acid monopotassium salt

Name: Phospho(enol)pyruvic acid monopotassium salt
Brand: SIGMA

≥97% (enzymatic), powder

Synonym(s):

2-(Phosphonooxy)-2-propenoic acid monopotassium salt, mono-Potassium phosphoenolpyruvate, PEP-K

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About This Item

Empirical Formula (Hill Notation):

C₃H₄KO₆P

CAS Number:

4265-07-0

Molecular Weight:

206.13

NACRES:

NA.32

PubChem Substance ID:

24898822

UNSPSC Code:

12352204

EC Number:

224-247-0

MDL number:

MFCD00044476

Beilstein/REAXYS Number:

4603446

Product Name

Phospho(enol)pyruvic acid monopotassium salt, ≥97% (enzymatic)

InChI key

SOSDSEAIODNVPX-UHFFFAOYSA-M

InChI

1S/C3H5O6P.K/c1-2(3(4)5)9-10(6,7)8;/h1H2,(H,4,5)(H2,6,7,8);/q;+1/p-1

SMILES string

[K+].OC(=O)C(=C)OP(O)([O-])=O

assay

≥97% (enzymatic)

form

powder

solubility

water: 100 mg/mL, clear, colorless

storage temp.

−20°C

Quality Level

200

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Application

Phospho(enol)pyruvic acid monopotassium salt has been used:

to maintain a constant concentration of adenosine triphosphate (ATP) for active gel assembly
as a component in assay buffer for in vitro ATPase assay
as a stock solution for optical trapping assay

Biochem/physiol Actions

Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by Pyruvate Kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

General description

Phospho(enol)pyruvic acid (PEP) acts as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase. It is a bifunctional carbohydrate, which exhibits antioxidant property. PEP may be a potential organ preservation agent in clinical transplantation.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000514564

0000472032

0000464200

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Alcoholic liver disease and the mitochondrial ribosome: methods of analysis.

Alan Cahill et al.

Methods in molecular biology (Clifton, N.J.), 447, 381-394 (2008-03-29)

Chronic alcohol consumption has been shown to severely compromise mitochondrial protein synthesis. Hepatic mitochondria isolated from alcoholic animals contain decreased levels of respiratory complexes and display depressed respiration rates when compared to pair-fed controls. One underlying mechanism for this involves

Single Molecule Analysis (2018)

Phosphoenolpyruvic acid, an intermediate of glycolysis, attenuates cellular injury induced by hydrogen peroxide and 2-deoxy-D-glucose in the porcine proximal kidney tubular cell line, LLC-PK1

Kondo Y, et al.

Journal of Health Science, 56(6), 727-732 (2010)

N-terminal polar amino acids of the C2 insert of nonmuscle myosin II-C2 regulate its functional properties

Saha S, et al.

Febs Letters, 590(23), 4223-4232 (2016)

Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone.

W J Bettger et al.

Proceedings of the National Academy of Sciences of the United States of America, 78(9), 5588-5592 (1981-09-01)

A serum-free, hormone-supplemented medium, enriched with a mixture of lipids, has been developed that supports rapid clonal growth of human diploid fetal lung fibroblasts (Flow 2000, WI-38, MRC-5, and IMR-90) and of low-passage human foreskin fibroblasts. The medium, which contains

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