SAB4301135
Anti-FLAG Tag antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
67
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
all
concentration
1.4 mg/mL
technique(s)
western blot: 1:1000-1:5000 (Cell Lysate)
isotype
IgG
immunogen sequence
DYKDDDDK conjugated to KLH (synthetic peptide)
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
General description
The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.
Immunogen
Synthetic peptide: DYKDDDDK conjugated to KLH
Application
Anti-FLAG Tag antibody produced in rabbit has been used in:
- western blotting
- co-immunoprecipitation
- immunofluorescence studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
The antibody detects transfected proteins contanining FLAG tag.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Xuan Yang et al.
Cancer science, 110(7), 2133-2144 (2019-05-09)
Lymphoid-specific helicase (LSH) is overexpressed in tumor tissues and its overexpression is associated with poor prognosis in several cancers. However, the role and molecular mechanism of LSH in hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that LSH was
Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
Cao Y, et al.
Biochimica et Biophysica Acta, 1863(12), 3148-3159 (2016)
Huimin Zhao et al.
Plant physiology, 183(3), 1026-1034 (2020-04-25)
Chromatin immunoprecipitation (ChIP) is the gold-standard method for detection of interactions between proteins and chromatin and is a powerful tool for identification of epigenetic modifications. Although ChIP protocols for plant species have been developed, many specific features of plants, especially
Jung-Ho Shin et al.
Nature communications, 7, 12612-12612 (2016-08-27)
Bacteria respond dynamically to the changes in zinc availability. Repression by the Bacillus subtilis transcription factor Zur requires Zn(II), which binds with negative cooperativity to two regulatory sites per dimer to form, sequentially, Zur2:Zn3 and Zur2:Zn4 forms of the repressor.
Haijie Li et al.
Journal of experimental & clinical cancer research : CR, 39(1), 12-12 (2020-01-15)
Histone lysine demethylase 4B (KDM4B) has been implicated in various pathological processes and human diseases. Glucose metabolism is the main pattern of energy supply in cells and its dysfunction is closely related to tumorigenesis. Recent study shows that KDM4B protects
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