U0508
Monoclonal Anti-Ubiquitin antibody produced in mouse
clone 6C1, ascites fluid
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About This Item
Conjugate:
unconjugated
Clone:
6C1, monoclonal
Application:
ARR, ELISA (i), WB
Citations:
37
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6C1, monoclonal
contains
15 mM sodium azide
species reactivity
human, rat, mouse, bovine
technique(s)
indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)
General description
Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.
Immunogen
purified bovine ubiquitin conjugated to KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Andrej Susor et al.
Reproduction (Cambridge, England), 134(4), 559-568 (2007-09-25)
In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis
Yuga Takahashi et al.
FEBS letters, 585(12), 1741-1747 (2011-05-17)
Nerve growth factor (NGF) binding to its receptor TrkA, which belongs to the family of receptor tyrosine kinases (RTKs), is known to induce its internalization, endosomal trafficking and subsequent lysosomal degradation. The Cbl family of ubiquitin ligases plays a major
Ana P Valencia et al.
BMC musculoskeletal disorders, 18(1), 436-436 (2017-11-11)
Rotator cuff (RTC) tears are a common clinical problem resulting in adverse changes to the muscle, but there is limited information comparing histopathology to contractile function. This study assessed supraspinatus force and susceptibility to injury in the rat model of