AB3150
Anti-β-Actin (ACTB) Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
32 kDa Fragment of beta-Actin
Sign In to View Organizational & Contract Pricing.
Select a Size
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Product Name
Anti-Fractin Antibody, CT, serum, Chemicon®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Analysis Note
Control
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
Application
Detect Fractin using this Anti-Fractin Antibody, C-terminus validated for use in WB, IC, IH(P).
Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.
General description
During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.
Immunogen
K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.
Physical form
Liquid whole rabbit serum containing no preservatives.
Preparation Note
Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Zhouheng Ye et al.
Journal of neuropathology and experimental neurology, 77(3), 260-265 (2018-02-07)
Our previous study demonstrated caspase independent DNA fragmentation after very brief cerebral ischemia, the mechanism of which was unclear. In this study, we explore whether actin is cleaved following focal cerebral ischemia, and whether these structural changes of actin might
Yohan van de Looij et al.
Annals of clinical and translational neurology, 1(12), 955-967 (2015-01-13)
Lactoferrin (Lf) is an iron-binding glycoprotein secreted in maternal milk presenting anti-inflammatory and antioxidant properties. It shows efficient absorption into the brain from nutritional source. Brain injury frequently resulting from cerebral hypoxia-ischemia (HI) has a high incidence in premature infants
Peter L Peng et al.
Neuron, 49(5), 719-733 (2006-03-01)
ADAR2 is a nuclear enzyme essential for GluR2 pre-mRNA editing at Q/R site-607, which gates Ca2+ entry through AMPA receptor channels. Here, we show that forebrain ischemia in adult rats selectively reduces expression of ADAR2 enzyme and, hence, disrupts RNA
Vanessa Ginet et al.
BioFactors (Oxford, England), 42(3), 323-336 (2016-06-18)
Lactoferrin (Lf), component of maternal milk, has antioxidant, anti-inflammatory and antimicrobial properties. Neuroprotective effects of Lf on the immature brain have been recently shown in rodent models of intrauterine growth restriction and cerebral hypoxia/ischemia. Here we postulated that Lf could
Isoflurane-induced apoptosis of neurons and oligodendrocytes in the fetal rhesus macaque brain.
Creeley, CE; Dikranian, KT; Dissen, GA; Back, SA; Olney, JW; Brambrink, AM
Anesthesiology null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB3150 | 04053252269257 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service