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Merck

88190

Tetrazolium Blue Chloride

≥90% (T), powder, suitable for microbiology

Synonym(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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About This Item

Empirical Formula (Hill Notation):
C40H32Cl2N8O2
CAS Number:
1871-22-3
Molecular Weight:
727.64
NACRES:
NA.83
PubChem Substance ID:
329769640
UNSPSC Code:
12352204
EC Number:
217-488-8
MDL number:
MFCD00040933
Beilstein/REAXYS Number:
3894077

Key Documents

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Product Name

Tetrazolium Blue Chloride, suitable for microbiology, ≥90% (T)

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

assay

≥90% (T)

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 0.5 g/10 mL ( hot)

application(s)

microbiology

Quality Level

200

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Other Notes

Used in the assay of reducing sugars; Used in the preparation of DNA-cation complexes, immunoadsorbents for anti-DNA antibodies.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5545
BCCM1219
BCCL2852
BCCL6545
BCCK1866

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V A DeBari et al.
Journal of immunological methods, 40(1), 89-94 (1981-01-01)
DNA can be precipitated as complexes with metal and organic cations. We have prepared these products using divalent metal cations (Cu2+, Mn2+, Ni2+, Zn2+, and Co2+) and ditetrazolium ions (blue tetrazolium and neotetrazolium). They are effective adsorbents, in vitro, for
J.P. Robin et al.
Sciences Des Aliments, 1, 233-233 (1981)
Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later.
Pannecouque C, Daelemans D, and De Clercq E
Nature Protocols, 3(3), 427-434 (2008)
Christophe Pannecouque et al.
Nature protocols, 3(3), 427-434 (2008-03-08)
Since its first description 20 years ago, the tetrazolium-based colorimetric (MTT) assay using MT-4 cells for the detection of anti-HIV compounds has been widely used. This method, which remains popular, provides more information than more recently developed methods and, therefore
Diana Binanti et al.
Medical mycology, 52(2), 196-201 (2013-07-19)
Pneumocystis carinii f. sp. suis is a fungus multiplying in the respiratory tract of pigs which occasionally is associated with interstitial pneumonia. Identification of Pneumocystis in tissue samples is considered difficult and there are only scarce data on its occurrence
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