A0856

Anti-ATG5 (N-terminal) antibody produced in rabbit

Name: Anti-ATG5 (N-terminal) antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, PBS solution

Synonym(s):

ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Atg5 Antibody, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09753847

Product Name

Anti-ATG5 (N-terminal) antibody produced in rabbit, affinity isolated antibody, PBS solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

PBS solution

mol wt

antigen ~56 kDa (Atg5-Atg12 complex)

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunofluorescence: suitable
western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells

UniProt accession no.

Q9H1Y0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Application

Anti-ATG5 (N-terminal) antibody has been used:

in immunoblotting
in immunofluorescence
in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Biochem/physiol Actions

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000467683

0000086427

0000199537

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Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis

Grootaert M O A J, et al.

Autophagy, 11(11), 2014-2032 (2015)

Herpes simplex virus type I induces the accumulation of intracellular β-amyloid in autophagic compartments and the inhibition of the non-amyloidogenic pathway in human neuroblastoma cells.

Soraya Santana et al.

Neurobiology of aging, 33(2), 430-430 (2011-01-29)

Mounting evidence suggests that herpes simplex virus type 1 (HSV-1) is involved in the pathogenesis of Alzheimer's disease (AD). Epidemiological analyses have shown that HSV-1 is a risk factor for AD in people with at least 1 type 4 allele

Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells

Seto S, et al.

PLoS ONE, 8(12), e86017-e86017 (2013)

Distinct Contributions of Autophagy Receptors in Measles Virus Replication.

Denitsa S Petkova et al.

Viruses, 9(5) (2017-05-23)

Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes

The deubiquitinating enzyme USP36 controls selective autophagy activation by ubiquitinated proteins.

Emmanuel Taillebourg et al.

Autophagy, 8(5), 767-779 (2012-05-25)

Initially described as a nonspecific degradation process induced upon starvation, autophagy is now known also to be involved in the degradation of specific ubiquitinated substrates such as mitochondria, bacteria and aggregated proteins, ensuring crucial functions in cell physiology and immunity.

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