A25120
DEAE–Sephadex® A-25 chloride form
Synonym(s):
DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®
form
powder
technique(s)
activity assay: suitable
matrix
cross-linked dextran
matrix active group
functional group
bead size
40-125 μm (dry)
pore size
~30,000 Da exclusion limit
operating pH
2-9
capacity
3-4 meq/g ion exchange capacity
compatibility
mode of use mixed weak and strong anion exchange chromatography
Quality Level
General description
The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
Application
DEAE-Sephadex® A-25 has been used:
- to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
- in the estimation of GLS content in Chinese kale sprouts
- production and purification of endozepine from astrocytes
- to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients
Features and Benefits
- Weak anion exchanger
- Suitable for batch techniques
- High binding capacities
- Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
Legal Information
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
A C Newton et al.
Analytical biochemistry, 156(1), 56-60 (1986-07-01)
The greatly enhanced fluorescence of Tb3+ when complexed with dipicolinic acid affords a simple and highly sensitive method for monitoring continuous anion flux through the erythrocyte anion transporter, band 3. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) is entrapped in human erythrocytes and
T M Parkinson
Journal of lipid research, 8(1), 24-29 (1967-01-01)
Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine. Of these substances, diethylaminoethyl (DEAE) cellulose, guanidoethyl cellulose, and DEAE Sephadex reduced hypercholesterolemia
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
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