A3809
Asparaginase from Escherichia coli
lyophilized powder, 100-300 units/mg protein (biuret)
Synonym(s):
L-Asparagine Amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-765-3
MDL number:
Specific activity:
100-300 units/mg protein (biuret)
form
lyophilized powder
specific activity
100-300 units/mg protein (biuret)
composition
Protein, ≥60%
storage temp.
2-8°C
Quality Level
General description
Asparaginase is a bacterial enzyme and also a chemotherapeutic drug.
Application
Asparaginase from Escherichia coli has been used:
- to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115
- as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues
- to elicit amino acid deprivation
Biochem/physiol Actions
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .
Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients.
Physical form
Lyophilized powder containing sodium chloride
Preparation Note
Chromatographically purified
Analysis Note
Protein determined by biuret.
Other Notes
One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.
signalword
Warning
hcodes
Hazard Classifications
Repr. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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L-Asparaginase: discovery and development as a tumor-inhibitory agent.
J D Broome
Cancer treatment reports, 65 Suppl 4, 111-114 (1981-01-01)
Antineoplastic Drugs
Pharmacology and Therapeutics for Dentistry, 530-562 (2017)
Changes in plasma angiotensin subtypes in Japanese eel acclimated to various salinities from deionized water to double-strength seawater
Wong MKS, et al.
General and Comparative Endocrinology, 178(2), 250-258 (2012)
Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling
Mukherjee A, et al.
Cellular and molecular gastroenterology and hepatology (2019)
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Hitzel J, et al.
Nature Communications, 9(2292), 1-18 (2018)
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