L5142
Lectin from Triticum vulgaris (wheat)
biotin conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
34058011
Form:
lyophilized powder
conjugate
biotin conjugate
Quality Level
form
lyophilized powder
composition
Protein, ≥80% E1%/280
extent of labeling
2-4 mol biotin per mol protein
storage temp.
2-8°C
General description
Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- in lectin binding assay of samples from intrauterine stages of filarial parasites
- in the histological studies of mice myocytes
- for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate
Preparation Note
Conjugates are prepared from affinity purified lectin.
Legal Information
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)