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Merck

N9876

4-Nitrophenyl butyrate

lipase substrate, chromogenic, esterase substrate, ≥98%, liquid

Synonym(s):

p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C10H11NO4
CAS Number:
2635-84-9
Molecular Weight:
209.20
NACRES:
NA.83
PubChem Substance ID:
24897915
UNSPSC Code:
12352204
MDL number:
MFCD00047734
Beilstein/REAXYS Number:
1879729

Product Name

4-Nitrophenyl butyrate, ≥98%

InChI

1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

SMILES string

CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI key

DVDUMIQZEUTAGK-UHFFFAOYSA-N

assay

≥98%

form

liquid

density

1.19 g/mL at 20 °C (lit.)

storage temp.

−20°C

Quality Level

200

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Application

4-nitrophenyl butyrate has been used:
  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase

General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000481866
0000481866
0000465581
0000465580
0000465581

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Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate
Pliego J, et al.
Sensors, 15(2), 2798-2811 (2015)
Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
Nathalia S Rios et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(9), 741-747 (2019-06-16)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h
Sara Arana-Peña et al.
Frontiers in bioengineering and biotechnology, 8, 36-36 (2020-03-18)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the objective of
Differential response to chemically altered polyethylene by activated mature human monocyte-derived macrophages
Xing S, et al.
Biomaterials, 23(17), 3595-3602 (2002)
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