P3243
Phosphodiesterase I from Crotalus adamanteus venom
vial of ≥100 units, Purified
Synonym(s):
5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-806-5
MDL number:
Specific activity:
≥20.0 unit/mg solid
Biological source:
Crotalus adamanteus venom
biological source
Crotalus adamanteus venom
Quality Level
form
solid
quality
Purified
specific activity
≥20.0 unit/mg solid
packaging
vial of ≥100 units
storage temp.
−20°C
Application
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.
Biochem/physiol Actions
Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.
Preparation Note
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
Other Notes
One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a