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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
6C5, monoclonal
Species reactivity:
frog, human, porcine, chicken, canine, rabbit, mouse, fish, rat
Application:
—
Citations:
426
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
6C5, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
frog, human, porcine, chicken, canine, rabbit, mouse, fish, rat
should not react with
bovine, yeast
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA
Immunoblotting (1-10 µg/mL;
Immunocytochemistry )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rabbit ... Gapdh(100009074)
General description
Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Immunogen
Rabbit
purified, rabbit muscle
Application


Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Analysis Note
Positive Control
Rat skeletal muscle
Rat skeletal muscle
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Karina M Cirone et al.
Cell and tissue research, 379(1), 207-217 (2019-09-04)
Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic diarrheic intestinal infections in domestic and wild ruminants (paratuberculosis or Johne's disease) for which there is no effective treatment. Critical in the pathogenesis of MAP infection is the invasion and survival into macrophages
Jessica L Binder et al.
PloS one, 15(3), e0230026-e0230026 (2020-03-26)
Pathological accumulation of microtubule associated protein tau in neurons is a major neuropathological hallmark of Alzheimer's disease (AD) and related tauopathies. Several attempts have been made to promote clearance of pathological tau (p-Tau) from neurons. Transcription factor EB (TFEB) has
Robert Sertori et al.
Journal of immunology (Baltimore, Md. : 1950), 196(1), 135-143 (2015-11-22)
The IL-2 receptor γ common (IL-2Rγc) chain is the shared subunit of the receptors for the IL-2 family of cytokines, which mediate signaling through JAK3 and various downstream pathways to regulate lymphopoiesis. Inactivating mutations in human IL-2Rγc result in SCID