50484
Methylene Blue solution
suitable for microbiology
Synonym(s):
Ehrlich’s reagent III, Loeffler’s Methylene Blue
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3641570
InChI
1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
InChI key
CXKWCBBOMKCUKX-UHFFFAOYSA-M
shelf life
limited shelf life, expiry date on the label
concentration
0.5% in H2O (0.5 g/100 mL)
technique(s)
microbe id | staining: suitable
application(s)
microbiology
suitability
Bacillus spp., Corynebacterium spp., bacteria
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General description
Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).
Other Notes
reagent contains:
methylene blue 0.5 g
distilled water 100 ml
methylene blue 0.5 g
distilled water 100 ml
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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