A8452
Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse
ascites fluid, clone C3
Synonym(s):
Afp Antibody, Afp Antibody - Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, Alpha Fetoprotein Antibody
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About This Item
Conjugate:
unconjugated
Clone:
C3, monoclonal
Application:
DB, ELISA (i), IHC (p)
Species reactivity:
pig, canine, human
Citations:
92
Technique(s):
dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver, indirect ELISA: suitable
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
C3, monoclonal
contains
15 mM sodium azide
species reactivity
pig, canine, human
should not react with
mouse, cat, rat, bovine
technique(s)
dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver, indirect ELISA: suitable
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... AFP(174)
Application
Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- dot blot
- immunocytochemistry
- immunofluorescence
- immunocytochemistry
Mouse Monoclonal Anti-α-Fetoprotein has been used for immunocytochemistry. The product can also be used for dot blot and indirect ELISA.
Biochem/physiol Actions
Increased concentrations of α-fetoprotein (AFP) in serum are less common in patients with malignancies of the gastrointestinal tract and of other organ systems with massive hepatic metastases. Hence, AFP determination is useful for the diagnosis of certain germ line tumors that contain yolk sac structures (testicular and ovarian tumors), hepatocellular carcinoma (hepatoma), and some malignant tumors of the gastrointestinal tract. AFP serves as an important marker for certain genetic and embryonic defects. Routine screening of pregnancies with a risk of neural tube defects (spina bifida, anencephaly, and Down′s syndrome) may be facilitated by the monitoring of AFP levels.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
α-Fetoprotein (AFP) is a one of the main glycoprotein present in the fetal liver, gastrointestinal tract, and yolk sac. AFP levels in amniotic fluid have been used as biomarkers for anencephaly and spina bifida.
AFP is a plasma protein found highly expressed in the human fetus where it functions to block the transport of estradiol across the placenta.
Monoclonal Anti-α-Fetoprotein (AFP) (mouse IgG2a isotype) is derived from the C3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human α-fetoprotein.
Immunogen
human α-fetoprotein
Physical form
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey
Shimozawa N, et al.
Reproduction (Cambridge, England), 139(3), 565-565 (2010)
Anne M Bara et al.
Stem cell research, 17(2), 441-443 (2016-11-24)
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein
Mark O Clements et al.
Tissue engineering, 12(7), 1741-1751 (2006-08-08)
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery. To achieve this goal, genetic tools need to be optimized and developed for controlling and manipulating
Alpha-fetoprotein in ontogenesis and its association with malignant tumors.
G I Abelev
Advances in cancer research, 14, 295-358 (1971-01-01)
Alpha-fetoprotein in the antenatal diagnosis of anencephaly and spina bifida.
D J Brock et al.
Lancet (London, England), 2(7770), 197-199 (1972-07-29)
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